2020 Genetic Code Expansion Workshop
July 23 – July 29, 2020
at the Agricultural and Life Science Building
Oregon State University in Corvallis, Oregon
Ryan Mehl, Unnatural Protein Facility, Oregon State University
Rick Cooley, Unnatural Protein Facility, Oregon State University
Kari van Zee, Unnatural Protein Facility, Oregon State University
John Perona, Portland State University
Hyo Sang Jang, Oregon State University
2020 Workshop Fee:
Academic: $1950 (Until April 6th) and $2150 (April 6-June 1st)
Industrial $2850 (Until April 6th) and $3150 (April 6-June 1st)
Advances in Genetic Code Expansion have changed the landscape of studies and uses of proteins in vitro and in vivo by enabling incorporation of hundreds of non-canonical amino acids site-specifically into proteins. Genetic Code Expansion is inherently a fusion of synthetic biology and chemical biology. Success in genetic code expansion requires 1) the addition of all the necessary translational components to an organism that allow it to efficiently produce a protein with new chemical functionality site-specifically encoded and 2) optimization of scientific studies of the chemically modified protein in vivo or in vitro. Overall, success with Genetic Code Expansion relies on proficiency in both synthetic biology and chemical biology.
This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize existing and emerging genetic code expansion technology. This workshop aims to accommodate users with a wide range of GCE experience and backgrounds. Participants will be grouped as Novice or Expert to ensure each receives training that best suits their needs. In their personal statement, applicants should indicate which group best describes their background in GCE and the laboratory training they wish to receive.
- Little or no prior experience in Genetic Code Expansion technologies
- Wish to incorporate non-canonical amino acids into control proteins (e.g. GFP) and a protein of interest but haven’t yet tried or haven’t yet been successful
- Intend to use well-established bacterial GCE systems:
- Traditional expression strains: BL21(DE3) derivatives, DH10b
- Commercially available expression vectors (pET, pBAD)
- Established GCE plasmids (pDule, pEVOL, pUltra)
- Non-canonical amino acid is commercially available
- Use of standard bacterial expression media
- Will bring to the workshop expression vectors for protein of interest
- Extensive consultation not required prior to workshop
- Have successfully used GCE technologies previously
- Wish to expand or evolve current GCE capabilities for a specific application
- Require troubleshooting challenges specific to their research or protein of interest
- Using non-standard GCE systems
- Specialized GCE expression strains (C321, B95(DE3), phospho-amino acid incorporation, hydroxy-tryptophan)
- Use of customized expression vectors and vector combinations
- Customized expression media
- Extensive consultation required prior to workshop to ensure participant has access to appropriate GCE tools
- Participants may be required to customize, modify or clone new expression vectors prior to workshop
- Expression strains may need to be obtained or developed
- Custom synthesis of ncAA
- Wish to incorporate non-canonical amino acids in eukaryotic systems
The lecture component will cover Novice and Expert topics. Lectures will be highly interactive and focus on how new genetic code expansion components are generated and attributes of orthogonality, efficiency, fidelity and permissivity are defined, measured and are conditionally dependent. The major applications now afforded by the new non-canonical amino acids will be discussed with a focus on the future of each field and its limitations.
1. GCE: When things don’t work, “the good, the bad, the ugly”
2. GCE Workshop Goals and Participants introduce their projects/research interests
3. Generation, selection and characterization of GCE components.
4. Categories of ncAA and their applications
5. tRNA synthetase catalytic function and mechanisms
6. Characterizing ncAA tRNA/synthetases
7. Practical considerations for success in prokaryotic ncAA production
8. Bioorthogonal ligations -overview.
9. Practical considerations for eukaryotes expanded codes
10. GCE for post-translational modifications
11. Library cloning and selections
March 1 the first round of acceptances and Scholarship awards will be announced
Early-registration pricing will be open to April 6, 2020
Applications will be accepted until June 1, 2020
Applicants should have a background in biochemistry and working knowledge of the basic laboratory techniques of protein expression. In the personal statement, applicants should specify:
1) their experience with genetic code expansion, 2) how they will apply genetic code expansion in their current projects or teaching, and 3) where they see genetic code expansion fitting in their career goals.
Financial Aid: Full scholarship and half scholarship support is available for the GCE workshop. The number of scholarships available depends on a number of factors, including the level of financial support in a given year and the number of students requesting stipends. Scholarships are awarded to support women and minorities in research and education. Scholarships will be provided to underwrite full or partial registration fees. Financial support for travel costs and ground transportation to and from OSU or lodging is not available for any applicant.
To apply for scholarship support, please upload a separate letter with your application materials that clearly states the amount you are requesting and provides a full justification for the request. The amount awarded will be based upon the availability of funding.
Workshop Selection Process: The number of places in the GCE workshop is strictly limited to ensure excellent instructor-to-student ratios and adequate access to laboratory equipment. Instructors are confronted with the difficult task of selecting diverse students from a large number of well-qualified applicants. To facilitate this process, each applicant is asked to submit two files during application/registration: a personal statement and a CV. In their personal statements, applicants should clearly describe their reasons for wanting to take this course. As part of this, applicants are encouraged to outline 1) their experience with genetic code expansion, 2) how they will apply genetic code expansion in their current projects or teaching, and 3) where they see genetic code expansion fitting in their career goals.
Workshop admission is based first and foremost on the degree to which an applicant would benefit from the training opportunity, which in turn is judged from the information provided by the applicant.
Workshop application instructions:
The online application for the Workshop requires three components-
1. Basic personal/contact information
2. A personal statement/essay, in 1000 words or less. Please tell the admissions committee why you are applying for the course, how you will benefit from taking it, and what you hope to gain. If there is a specific GCE problem you are planning on resolving please provide a brief description here
3. Curriculum Vitae
4. Optional Component: Financial aid letter that clearly states the amount you are requesting and provides a full justification for the request
- Application vs. Registration: Completing an application will not register you to attend, nor does it guarantee acceptance to attend. Registration can only be completed once you have been accepted by the Conference Chair/Committee. If accepted you will be notified via email with an unpublished link to register for the conference. Registration and payment arrangements are expected at that time.
- Payments: Payment in full (less any financial support awarded) is required six (6) weeks prior to the start of the course. Payment will not be accepted until you have completed the registration process.
- Financial Aid: We understand that your acceptance is sometimes conditional on the amount of financial aid provided to you.
- Wait List: We anticipate the workshop to be full before the deadline. If the workshop is oversubscribed you will be placed on a waiting list. Applications will be considered at the discretion of the workshop chairs. One application per individual.
- Acceptance/Rejection: You will be advised by email approximately eight weeks prior to the start of the workshop whether your application has been accepted. If accepted, the link you will be provided for registration is an unpublished link. You then have a limited time to accept the offer to participate in the Workshop. We understand that your acceptance is sometimes conditional on the amount of financial aid provided to you.Payments: Payment in full (less any financial support awarded) is required six weeks prior to the start of the course. Please do NOT send payment until you are officially accepted into the workshop. Instructions on how to arrange payment will be included in your acceptance email.
Location On OSU Campus
Agricultural and Life Science Building:
Workshop lectures and laboratory sessions will be held in the Ag and Life Sciences Building (ALS) 2750 SW Campus Way http://oregonstate.edu/campusmap/
ALS rooms 2018 (lectures, coffee), 0023 (teaching laboratory), 2009A (lunches), 2124 (Synthetic Biology Core facility), Main Office 2011